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Surgical setup (A) Animal is submerged in a container filled with Tricaine solution. Also pictured is a timer and an example surgical tray. (B) Once the animal is anesthetized, it is laid on its side on a surgery tray covered with moistened kimwipes, then covered with moistened kimwipes and kept moist by frequently misting with Working Holfreter’s Solution. (C) The animal is placed under the stereomicroscope with gooseneck lamps for surgery. The required surgical tools for nerve-deviation surgery – 2 #5 watchmaker forceps and a pair of <t>micro-dissecting</t> (Noyes) scissors – are also pictured. (D) The animal is placed on and covered with ice after surgery. The targeted limb and wound site should remain exposed to prevent disruption of the surgery site and frequently misted with Working Holfreter’s Solution.
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Surgical setup (A) Animal is submerged in a container filled with Tricaine solution. Also pictured is a timer and an example surgical tray. (B) Once the animal is anesthetized, it is laid on its side on a surgery tray covered with moistened kimwipes, then covered with moistened kimwipes and kept moist by frequently misting with Working Holfreter’s Solution. (C) The animal is placed under the stereomicroscope with gooseneck lamps for surgery. The required surgical tools for nerve-deviation surgery – 2 #5 watchmaker forceps and a pair of micro-dissecting (Noyes) scissors – are also pictured. (D) The animal is placed on and covered with ice after surgery. The targeted limb and wound site should remain exposed to prevent disruption of the surgery site and frequently misted with Working Holfreter’s Solution.

Journal: STAR Protocols

Article Title: Protocol for patterning competency assay in regenerative axolotl limb cells using the competency accessory limb model

doi: 10.1016/j.xpro.2025.103920

Figure Lengend Snippet: Surgical setup (A) Animal is submerged in a container filled with Tricaine solution. Also pictured is a timer and an example surgical tray. (B) Once the animal is anesthetized, it is laid on its side on a surgery tray covered with moistened kimwipes, then covered with moistened kimwipes and kept moist by frequently misting with Working Holfreter’s Solution. (C) The animal is placed under the stereomicroscope with gooseneck lamps for surgery. The required surgical tools for nerve-deviation surgery – 2 #5 watchmaker forceps and a pair of micro-dissecting (Noyes) scissors – are also pictured. (D) The animal is placed on and covered with ice after surgery. The targeted limb and wound site should remain exposed to prevent disruption of the surgery site and frequently misted with Working Holfreter’s Solution.

Article Snippet: 125 mm dissecting scissors , Indigo Instruments , 22212.

Techniques: Disruption